PMID: Vet World. 2021 Aug ;14(8):2206-2211. Epub 2021 Aug 25. PMID: 34566340 Abstract Title: Antibacterial activity of clove () and cinnamon () essential oil against extended-spectrumβ-lactamase-producing bacteria. Abstract: Background and Aim: Extended-spectrumβ-lactamase (ESBL) is an enzyme produced by the family of, especiallyand, which can hydrolyzeβ-lactam antibiotics, such as penicillins, cephalosporins, cephamycin, and carbapenem. ESBL-producing bacteria are widely distributed from farms to slaughterhouses until food products originating from animals are available in the market, which plays an important role as a pathway for the exposure and transmission of ESBL-producing bacteria from food products of animal origin to humans. This study aimed to determine the antibacterial activity of(clove) and(cinnamon) essential oils against strains resistant to ESBL-producingandisolates.Materials and Methods: The antibacterial activity of clove and cinnamon essential oils was tested against three strains of tested bacteria using the disk diffusion method. The minimum inhibitory concentration (MIC) of clove and cinnamon essential oils was determined using the broth microdilution method. The minimumbactericidal concentration (MBC) was determined using the MIC. Morphological changes on each tested bacteria were observed through scanning electron microscopy (SEM).Results: Both essential oils exhibited inhibitory effects toward all test organisms, indicated by inhibition zones around the disk. The MIC values of clove essential oil were 0.078% (v/v) for all tested bacteria, whereas the MICs of cinnamon essential oil ranged from 0.039% (v/v) to 0.156% (v/v) for all tested bacteria. MBC values of clove and cinnamon essential oils ranged from 0.078% (v/v) to 0.156% (v/v) for all tested bacteria. There were morphological changes in each tested bacterial cell that was observed through SEM. Each tested bacteria treated with clove and cinnamon essential oils showed shrinkage and cells lysis.Conclusion: It was concluded that clove and cinnamon essential oils have emerged as effective antibacterial agents by showing high antibacterial activity against ESBL-producingandisolates, as evidenced by the inhibition zone diameter and MIC value.
read more
read more