PMID: PeerJ. 2021 ;9:e12398. Epub 2021 Nov 15. PMID: 34820178 Abstract Title: Fighting SARS-CoV-2 with green seaweedsp. extract: extraction protocol predetermines crude ulvan extract anti-SARS-CoV-2 inhibition properties inVero-E6 cells assay. Abstract: Due to the global COVID-19 pandemic, there is a need to screen for novel compounds with antiviral activity against SARS-COV-2. Here we compared chemical composition and the anti- SARS-COV-2 activity of two differentsp. crude ulvan extracts: one obtained by an HCl-based and another one by ammonium oxalate-based (AOx) extraction protocols. The composition of the crude extracts was analyzed and their antiviral activity was assessed in a cytopathic effect reduction assay using Vero E6 cells. We show that the extraction protocols have a significant impact on the chemical composition, anti- SARS-COV-2 activity, and cytotoxicity of these ulvan extracts. The ulvan extract based on the AOx protocol had a higher average molecular weight, higher charge, and 11.3-fold higher antiviral activity than HCl-based extract. Our results strongly suggest that further bioassay-guided investigation into bioactivity of compounds found insp. ulvan extracts could lead to the discovery of novel anti-SARS-CoV-2 antivirals.
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