PMID: Neurotoxicology. 2021 Nov 20. Epub 2021 Nov 20. PMID: 34813867 Abstract Title: Fine particulate matter induces endoplasmic reticulum stress-mediated apoptosis in human SH-SY5Y cells. Abstract: Exposure to ambient fine particulate matter (PM) may contribute to brain injury, however, the molecular mechanisms have not yet been fully described. In this study, the human SH-SY5Y cells were treated with PMwith different concentrations (0, 25, 100, and 250μg/mL) for 24 h to investigate the cell apoptosis mediated by endoplasmic reticulum (ER) stress. The ratio of apoptosis, Calevel, biomarkers of ER stress and apoptosis were determined. The results revealed that PMtriggered the increase of apoptosis ratio and cellular Calevels. Compared with control, the expression of GRP78 and phosphorylation of IER1α and p38 were enhanced significantly in the cells under the conditions of PMexposure for activating ER stress signals. Besides, the key genes (CHOP/DR5/Caspase8/Caspase12) in ER stress-induced apoptosis signals were up-regulated after the PMtreatment compared to the control. The results suggested PMinduced apoptosis in SH-SY5Y cells by the stimulation of ER stress, which may be the potential mechanism of neurological diseases incurred by PM.
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