PMID: Pharm Biol. 2021 Dec ;59(1):1314-1325. PMID: 34569428 Abstract Title: SIRT 3 was involved inseed oil protection testis from oxidative stress:andanalyses. Abstract: CONTEXT: L. (Solanaceae) seed oil (LBSO) exerts LBSO exerts protective effects in the testisandvia upregulating SIRT3.OBJECTIVE: This study evaluates the effects and mechanism of LBSO in the d-galactose (d-gal)-induced ageing testis.MATERIALS AND METHODS: Male Sprague Dawley (SD) rats ( = 30, 8-week-old) were randomly divided into three groups: LBSO group ( = 10) where rats received subcutaneous injection of d-gal at 125 mg/kg/day for 8 weeks and intragastric administration of LBSO at 1000 mg/kg/day for 4 weeks, ageing model group ( = 10) received 8-week-sunbcutaneous injection of d-gal, and control group ( = 10) with same administration of normal saline. Lentivirus had established TM4 cells with SIRT3 overexpression or silencing before LBSO intervened.RESULTS: Treatment with LBSO, the levels of INHB and testosterone both increased, compared to ageing model., we found the EDof LBSO was 86.72 ± 1.49 and when the concentration of LBSO at 100 μg/mL to intervene TM4 cells, the number of cells increased from 8120 ± 676.2 to 15251 ± 1119, and the expression of SIRT3, HO-1, and SOD upregulated. However, HO-1 and SOD were dysregulated by silencing SIRT3. On the other hand, the expression of AMPK and PGC-1upregulated as an effect of SIRT3 overexpression by lentivirus, meanwhile the same increasing trend of that being found in cells treated with LBSO, compared to control group.DISCUSSION AND CONCLUSIONS: LBSO alleviated oxidative stress in d-gal-induced sub-acutely ageing testis and TM4 cells by suppressing the oxidative stress to mitochondria via SIRT3/AMPK/PGC-1α.
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