PMID: Tissue Cell. 2021 Nov 25 ;74:101697. Epub 2021 Nov 25. PMID: 34923198 Abstract Title: Vitamin Bameliorate Tramadol-induced oxidative stress, endocrine imbalance, apoptosis and NO/iNOS/NF-κB expression in Sprague Dawley rats through regulatory mechanism in the pituitary-gonadal axis. Abstract: This study aimed at the effect of vitamin B(VB) on tramadol (TRM) induced pituitary-gonadal Axis toxicity. Thirty-two (32) adult male rats were randomized into four groups of eight (n = 8) rats each. Group A served as control was given 1 mL normal saline, group B received 50 mg /kg bwt TRM, group C received 0.5 mg/kg bwt VBand group D received 50 mg /kg bwt TRM and 0.5 mg/kg bwt VBthrough gastric gavage daily for 8 weeks. Parameters tested include sperm parameter, male reproductive hormone, testicular histology, glucose, lactate dehydrogenase (LDH), acid phosphate (ACP), and alkaline phosphate (ALP) activity, steroidogenic protein, cytochrome P450 A1, nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), nuclear factor- kappa B (NF-κB), oxidative and antioxidant makers. Tramadol significantly decreases sperm quality, hormone, steroidogenic protein, cytochrome P450 A1, ACP, ALP, and increases glucose, LDH, oxidative stress, mtTFA,and UCP2, p53 expression, NO, iNOS, NF-κB, IL-1β, IL-6, TNF-α, and caspase-3 activity. Degenerative alterations of the testes' and pituitary architecture and perturbation of spermatogenesis were observed in TRM-treated rats. The intervention of VBdownregulated testicular oxidative stress, inflammatory markers, glucose, lactate, LDH, p53, caspase-3, mtTFA, and UCP2. And upregulate antioxidant, sperm quality, hormone, and spermatogenic cells. Vitamin Bexhibited mitigation against TRM-induced testicular dysfunction via its antioxidant, anti-inflammatory and anti-apoptotic effects.
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